Comparative characteristics of the antiphlogistic activity of cryoextracts of biological tissues and the conditioned medium of mesenchymal stem cells on the model of autoimmune arthritis
DOI:
https://doi.org/10.32782/2415-8127.2024.69.9Keywords:
cell-free cryopreserved biological agents, autoimmune diseases, spleen cryoextract, placenta cryoextract, mesenchymal stem cell conditioned mediumAbstract
Introduction. Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by progressive symmetrical inflammatory joint damage that leads to cartilage destruction and bone erosion. Deformation and destruction of joints in RA can lead to irreversible physical disabilities that impair the quality of life of patients and can serve as a basis for exacerbation of concomitant diseases and cause early death. Social problems associated with RA include increased socioeconomic burden, reduced work capacity, and reduced social adaptation. The purpose of the study is to conduct a comparative assessment of the anti-inflammatory activity (AIA) of cryoextracts of the placenta (СEP) and spleen (CES) and the conditioned medium of mesenchymal stem cells (CM-MSC) on a model of experimental autoimmune arthritis. Materials and methods. Experimental studies were conducted on 42 male shuras weighing 200–220 g in accordance with the basic bioethical norms of the Helsinki Declaration of the World Medical Association. Adjuvant arthritis (AA) was modeled by subplantar injection of complete Freund’s adjuvant (CFA) into the hind right limb at the rate of 0.1 ml per rat. On «0» (initial indicators), 14 and 28 days of the experiment, the development of the inflammatory reaction was evaluated by the dynamics of the volume of the limb (in ml). Research results and their discussion. The conducted study showed that the introduction of CFA led to a statistically significant (p=0.009) increase in the volume of the affected limb on the 14th day of the experiment by 2 times or more (from +100.9% to +124.5%) in all animals with simulated AA, relative to the initial indicators. The studied cell-free cryopreserved biological agents have anti-inflammatory activity in the model of adjuvant arthritis in rats, which was indicated by a decrease in the volume of the damaged limb on the 28th day of the experiment relative to the indicators on the 14th day, respectively, by 25.1% when using CEP, by 20.0% when application of CES and by 22.0% when applying CM-MSK. Conclusions. According to the amount of anti-inflammatory activity in experimental rheumatoid arthritis, the investigated cell-free cryopreserved biological agents can be arranged in the following sequence: CEP (AIA=56.5%) ˃ CM-MSC (AIA=47.3%) ˃ CES (AIA=43.3%).
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