Morphofunctional characteristics of the intermediate areas of the greater omentum of white rats
DOI:
https://doi.org/10.32782/2415-8127.2023.67.12Keywords:
greater omentum, milky spots, serous-reticular membranes, vascular-fatty arcades, white rats.Abstract
In a human and a laboratory animal (white rats) the greater omentum has a similar structure and consists of two different tissues: one of which is represented by adipose tissue, and the other, which is the object of our work, is translucent and membranous – poorly vascularized and contains numerous fenestrations of different sizes. Objective. To study the morphofunctional structure of the intermediate areas of the greater omentum of white rats. Materials and methods. The experiment involved 20 white male rats of reproductive age, weighing from 278 to 346 grams. The material was intermediate areas of total preparations of the greater omentum, stained with hematoxylin-eosin and Van Gieson solutions, as well as similar preparations after intraperitoneal injection of activated carbon suspension. Results. During a detailed study of the total preparations of the greater omentum of white rats, it was established that the areas that occupy an intermediate position in the greater omentum between the vascular-fatty arcades are the thinnest, openwork-looking formations, woven from various wide and long looped cords, interlace with each other and limit different apertures. Considering the fact that in the greater omentum, such formations occupy an intermediate position between its vascular-fatty arcades, we consider it justified to assign them the name of serous-reticulate membranes. Each of them can be represented as a finely perforated, thinnest duplication of the peritoneum, in the meshlike structure of which one can distinguish, surrounded by mesothelium, relatively wide looped cords, and narrow, changing in configuration, membranes that transversely connect them. Conclusion. Serous-reticular membranes of the greater omentum perform a number of basic functions. First of all, they have a connecting role between vascular-fatty arcades, they also have contractile properties, and in addition, they are structurally adapted to the drainage of peritoneal fluid.
References
Huyghe S, de Rooster H, Doom M, Van den Broeck W. The Microscopic Structure of the Omentum in Healthy Dogs: The Mystery Unravelled. Anatomia, histologia, embryologia. 2016;45(3):209-18 DOI: 10.1111/ahe.12189.
Bilgiç T, İnce Ü, Narter F. Autologous omentum transposition for regeneration of a renal injury model in rats. Military Medical Research. 2022;9(1):1 DOI: 10.1186/s40779-021-00361-0.
Cleypool CGJ, Schurink B, van der Horst DEM, Bleys R. Sympathetic nerve tissue in milky spots of the human greater omentum. Journal of anatomy. 2020;236(1):156-64 DOI: 10.1111/joa.13077.
Shah S, Lowery E, Braun RK, Martin A, Huang N, Medina M, et al. Cellular basis of tissue regeneration by omentum. PloS one. 2012;7(6):e38368 DOI: 10.1371/journal.pone.0038368.
Directive 2010/63/EU of the European Parliament and of the Council on the protection of animals used for scientific purposes. 2010:276:0033:0079.
Hryn VH, Brovarnyk YAO, inventors. Higher State Education Institution “Ukrainian Medical Stomatological Academy”, assignee. Operating and preparation table with fixators for laboratory rats. Ukrainian patent № 142955. 2020 lyp. 10. http:// repository.pdmu.edu.ua/bitstream/123456789/13459/1/H_B_patent_2020.pdf. (Ukrainian).
Pellicciaro M, Vella I, Lanzoni G, Tisone G, Ricordi C. The greater omentum as a site for pancreatic islet transplantation. CellR4-- repair, replacement, regeneration, & reprogramming. 2017;5(3).
Maksymenko O. Strukturna orhanizatsiya sudynnozhyrovykh arkad velykoho cheptsya bilykh shchuriv [Structural organization of the vascular-fatty arcades of the greater omentum of white rats]. Morphologia. 2022;16(3):61-8. Ukrainian. DOI: https://doi.org/10.26641/1997-9665.2022.3.61-68
Wang ZB, Li M, Li JC. Recent advances in the research of lymphatic stomata. Anatomical record (Hoboken, NJ : 2007). 2010;293(5):754-61 DOI: 10.1002/ar.21101.
Wang AW, Prieto JM, Cauvi DM, Bickler SW, De Maio A. The Greater Omentum–A Vibrant and Enigmatic Immunologic Organ Involved in Injury and Infection Resolution. Shock. 2020;53(4):384-90 DOI: 10.1097/shk.0000000000001428.
Maksymenko O, Hryn V, Kostylenko Y. Zahalʹnyy plan budovy ta pryntsypy morfometrychnoho analizu velykoho cheptsya bilykh shchuriv. [General plan of the structure and principles of morphometric analysis of the greater omentum of white rats] APMM. 2022;22(1):105-10. Ukrainian. DOI: 10.31718/2077-1096.22.1.105.
Li S, Li Q. Cancer stem cells and tumor metastasis (Review). Int J Oncol. 2014;44(6):1806-12 DOI: 10.3892/ijo.2014.2362.
Miura M, Y. Y. Morphological study of human omental milky spots and their morphological changes in omental disseminated metastasis. Jap J Lymphology. 2011;34:2-6.
Meza-Perez S, Randall TD. Immunological Functions of the Omentum. Trends in immunology. 2017;38(7):526-36 DOI: 10.1016/j.it.2017.03.002.
Cranshaw ML, Leak LV. Milky spots of the omentum: a source of peritoneal cells in the normal and stimulated animal. Archives of histology and cytology. 1990;53 Suppl:165-77 DOI: 10.1679/aohc.53.suppl_165.
Liu Y, Hu J-n, Luo N, Zhao J, Liu S-c, Ma T, et al. The Essential Involvement of the Omentum in the Peritoneal Defensive Mechanisms During Intra-Abdominal Sepsis. Frontiers in Immunology. 2021;12 DOI: 10.3389/fimmu.2021.631609.
Liu M, Silva-Sanchez A, Randall TD, Meza-Perez S. Specialized immune responses in the peritoneal cavity and omentum. Journal of leukocyte biology. 2021;109(4):717-29 DOI: 10.1002/jlb.5mir0720-271rr.
Krishnan V, Tallapragada S, Schaar B, Kamat K, Chanana AM, Zhang Y, et al. Omental macrophages secrete chemokine ligands that promote ovarian cancer colonization of the omentum via CCR1. Communications Biology. 2020;3(1):524 DOI: 10.1038/s42003-020-01246-z.
Schurink B, Cleypool CGJ, Bleys RLAW. A rapid and simple method for visualizing milky spots in large fixed tissue samples of the human greater omentum. Biotechnic & Histochemistry. 2019;94(6):429-34 DOI: 10.1080/10520295.2019.1583375.
Etzerodt A, Moulin M, Doktor TK, Delfini M, Mossadegh-Keller N, Bajenoff M, et al. Tissue-resident macrophages in omentum promote metastatic spread of ovarian cancer. Journal of Experimental Medicine. 2020;217(4) DOI: 10.1084/ jem.20191869.