Microbiological justification of the use of xenotransplants saturated with silver nanocrystals for the treatment of burn wounds
Keywords:
burns, xenograft, silver nanocrystals, antimicrobial properties.Abstract
One of the main factors determining the prognosis of burn disease is microbial contamination of the wound. A colonization of the wound surface by microorganisms slows down its healing, leads to their deepening, and is also a source of generalization of the infection. It has been proven that the use of silver preparations increases the healing of wounds, in particular burns, due to the reduction of inflammatory processes in the wound, the prevention of its infection. These data formed the basis for a new therapeutic way of wound treatment in clinical practice. The aim of the study. To study the antimicrobial efficacy of xenografts satureted by silver nanocrystals, which may be applied in the treatment of burned wounds. Materials and methods. The antimicrobial efficacy of xenografts saturated with silver nanocrystals was investigated in vitro by diffusion into agar, in a liquid nutrient medium and by studying the adhesive activity using test cultures: Staphylococcus aureus АТСС 6538, Escherichia coli АТСС 25922, Pseudomonas aeruginosa АТСС 9027 та Candida albicans АТСС 885-653. Results. The antimicrobial properties of silver, which was saturated the pieces of cryolyophilized xenoskin, were not inferior to the effectiveness of modern dressings, which were used as a positive control (wound dressing applications Mepilex Transfer Ag and Atrauman Ag) in studies. Nanosilver had reduced the adhesive potential of microorganisms, which is important to prevent contamination of burn wounds. Conclusions. Thus, the possibility of using xenografts saturated with silver nanocrystals it is considered for local treatment of burns in order to prevent purulent-inflammatory complications that may occur.
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